Spin membrane immunoassay for use in meningococcal serology.
نویسندگان
چکیده
A modified and improved spin membrane immunoassay has been developed for detecting complement-activating antibodies to Neisseria meningitidis capsular polysaccharide antigens. The polysaccharides were incorporated in the membranes of large unilamellar vesicles prepared by the reverse-phase evaporation method and filled with the water-soluble spin label tempocholine chloride. Upon addition of group-specific antisera and complement, the lipid membrane was damaged and the spin label leaked out. This process was monitored by electron spin resonance spectroscopy. A satisfactory assay was developed for polysaccharides of group A and C, whereas in the case of the B system the assay was more labile. The method is rapid and has a sensitivity comparable to that of radioimmunoassay. When studying paired sera from five recruits vaccinated with an A + C polysaccharide vaccine, significant rises in titers to both A and C polysaccharides were observed in all the postvaccination sera.
منابع مشابه
Expression of Neisseria meningitidis iron-regulated outer membrane proteins, including a 70-kilodalton transferrin receptor, and their potential for use as vaccines.
The iron-regulated proteins (IRPs) of five group B meningococcal strains expressing class 2 outer membrane proteins were compared with those of five strains expressing class 3 proteins. Three to four high-molecular-weight IRPs were expressed by each strain, but their molecular sizes varied between strains and were not related to class 2 or 3 protein expression. Transferrin and hemoglobin could ...
متن کاملEfficient Immobilised TiO2 in Polyvinylidene fluoride (PVDF) Membrane for Photocatalytic Degradation of Methylene Blue
Immobilised titanium dioxide (TiO2) in membrane structures has recently become attractive. This is due to the elimination of the separation step after the process of photocatalytic degradation. The efficiency of the TiO2 surface area exposed to UV light as the main important parameter needs to be considered. The immobilisation of TiO2 nanoparticles in the polyvinylidene fluoride (PVDF) membrane...
متن کاملMucosal immune responses to meningococcal conjugate polysaccharide vaccines in infants.
BACKGROUND Serogroup C meningococcal conjugate polysaccharide vaccines have been reported to induce significant serum IgG antibodies and immunologic memory in infants. Because meningococcus is a mucosal pathogen colonizing the nasopharynx, local mucosal immune responses may play an important role in host defense against infection and carriage. We have investigated the mucosal IgA and IgG antibo...
متن کاملIn Silico Studies of Outer Membrane of Neisseria Meningitidis PorA: Its Expression and Immunogenic Properties
Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from ser...
متن کاملComparison of stool immunoassay with standard methods for detecting Helicobacter pylori infection.
Helicobacter pylori is the cause of type B gastritis and associated with peptic ulcer disease. Various methods are available for detecting H pylori, but all have limitations. H pylori infection can be diagnosed by tests requiring endoscopy (rapid urease test, histology, culture) and by non-invasive tests (carbon-13 urea breath test, serology, stool tests). The urea breath test is currently the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 18 4 شماره
صفحات -
تاریخ انتشار 1983